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KMID : 1094720070120020121
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 2 p.121 ~ p.124
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Hydrolysis of olive oil catalyzed by surfactant-coated Candida rugosa lipase in a hollow fiber membrane reactor
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Song Bao-Dong
Ding Hui
Wang Shi-Chang
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Abstract
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In this study, we invetigated the hydrolysis of olive oil catalyzed by a surfactant-coatedCandida rugosa lipase in a hydrophilic polyacrylonitrile hollow fiber membrane reactor and then compared the results to those using the native lipase. The organic phase was passed through the hollow inner fibers of the reactor and consisted of either the coated lipase and olive oil dissolved in isooctane or the coated lipase dissolved in pure olive oil. The aqueous phase was pumped through the outer space. After 12 h and with conditions of 30°C, 0.12 mg enzyme/mL and 0.62 M olive oil, the substrate conversion of the coated lipase reached 60%. This was twice the conversion for the same amount of native lipase that was pre-immobilized on the membrane surface. When using pure olive oil, after 12 h the substrate conversion of the coated lipase was 50%. which was 1.4 times higher than that of the native lipase.
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KEYWORD
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Candida rugosa, hydrolysis, lipase, membrane reactor, organic-aqueous two-phase, surfactant
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